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Novel Identification in Diverse Sample Types Through Direct Amplification

Integrated DNA Technologies (IDT) via YouTube

Overview

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Explore the benefits and data of extraction-free PCR for target identification in diverse sample types through this 27-minute conference talk. Discover IDT's one-step master mix that enables direct amplification from sample collection to PCR, eliminating traditional extraction steps. Examine the improved inhibitor resistance against heparin, hematin, and humic acid. Learn about efficient SARS-CoV-2 identification using qPCR products from IDT. Compare amplification results of viral analytes from extracted and non-extracted human samples. Investigate direct amplification of bacterial specimens in human urine and infectious pathogens from human rectal swabs. Analyze the performance of IDT's master mix against other commercially available options. Explore applications in plant genetic marker amplification from leaf punch samples and genotyping of resistance and susceptibility markers. Examine direct amplification of target cDNA from HEK293 cells for gene expression assays. Investigate the effects of lysis conditions on amplification using IDT's master mix. Gain insights into the PrimeTime Multiplex Dye Selection Tool for optimizing your experiments.

Syllabus

Intro
IDT PrimeTime One-Step 4X Broad-Range Master Mix
Better inhibitor resistance: heparin, hematin, humic acid
Efficient SARS-CoV-2 identification using qPCR products from IDT-primers, probes, and master mix
Direct amplification of viral specimens in human
IDT Prime Time One-Step 4X Broad-Range Master Mix results in comparable amplification of viral analytes from extracted and non-extracted human samples
Direct amplification of bacterial specimens in human urine using Prime Time One-Step Broad-Range Master Mix
Amplification of infectious pathogens from human rectal swabs
Amplification from human rectal swabs-amplification with IDT's master mix outperforms other commercially available master mix with extracted samples
Direct amplification of plant genetic markers from leaf punch samples
Better genotyping with direct amplification of resistance and susceptibility markers from plant leaf punches
Direct amplification of target cDNA from HEK293 cells for gene expression assays
The effects of lysis conditions on amplification using IDT'S Prime Time One-Step 4x Broad-Range Master Mix
PrimeTime Multiplex Dye Selection Tool

Taught by

Integrated DNA Technologies (IDT)

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