Novel Identification in Diverse Sample Types Through Direct Amplification
Integrated DNA Technologies (IDT) via YouTube
Overview
Syllabus
Intro
IDT PrimeTime One-Step 4X Broad-Range Master Mix
Better inhibitor resistance: heparin, hematin, humic acid
Efficient SARS-CoV-2 identification using qPCR products from IDT-primers, probes, and master mix
Direct amplification of viral specimens in human
IDT Prime Time One-Step 4X Broad-Range Master Mix results in comparable amplification of viral analytes from extracted and non-extracted human samples
Direct amplification of bacterial specimens in human urine using Prime Time One-Step Broad-Range Master Mix
Amplification of infectious pathogens from human rectal swabs
Amplification from human rectal swabs-amplification with IDT's master mix outperforms other commercially available master mix with extracted samples
Direct amplification of plant genetic markers from leaf punch samples
Better genotyping with direct amplification of resistance and susceptibility markers from plant leaf punches
Direct amplification of target cDNA from HEK293 cells for gene expression assays
The effects of lysis conditions on amplification using IDT'S Prime Time One-Step 4x Broad-Range Master Mix
PrimeTime Multiplex Dye Selection Tool
Taught by
Integrated DNA Technologies (IDT)