Method Optimization and High-Throughput Purification for mRNA and rAAV

Learn about downstream processing optimization for virus particles, vesicles, RNAs, plasmids, and DNA in this 26-minute webinar presented by molecular biologist Marko Narobe. Discover how to reduce development time through simultaneous condition screening using CIM® monolithic plates, which enable fast processes without shaking or incubation requirements. Explore the screening of mobile phases for rAAV capture step optimization to increase process productivity and product purity, while improving polishing steps. Examine strategies for enhancing dynamic binding capacity of mRNA on Oligo dT, with insights on achieving binding capacity of less than 6 mg/mL. Gain practical knowledge on scaling up optimized conditions to preparative scale chromatography and implementing screening tools for process development optimization using CIM® (Convective Interaction Media) monolithic chromatography products in gene therapy and vaccine applications.