Explore a detailed InDepth session from the SEB Annual Conference 2021 focusing on the targeting of tail-anchored membrane proteins to the inner nuclear envelope in plants. Delve into Norman Groves' research on nuclear localization signals and their role in protein trafficking. Learn about experimental questions, methods for measuring nuclear envelope enrichment, and the working model of NLS-mediated INM protein trafficking. Gain insights into the rules for nuclear envelope targeting and the sufficiency of monopartite NLS in enriching artificial membrane proteins at the nuclear envelope.
A Nuclear Localization Signal Targets Tail-Anchored Membrane Proteins to the Inner Nuclear Envelope in Plants
Society for Experimental Biology via YouTube
Overview
Syllabus
Intro
Soluble proteins are actively imported into the nucleus, through binding of the NLS
How do proteins traffic to the Inner Nuclear Membrane?
Models for membrane protein transport to the INM
Transport-Factor Mediated Trafficking of inner nuclear membrane proteins
Experimental Questions
Targeting a tail anchored ER protein to the nuclear envelope
Measuring NE enrichment using nuclear localization index NLI
Addition of an NLS to an chimeric membrane protein results in NE enrichment
Defining Rules for NE Targeting
NLS fused chimeric membrane proteins access
Working model of NLS-mediated INM protein trafficking
Addition of a monopartite NLS is sufficient to enrich an artificial membrane protein at the NE
Taught by
Society for Experimental Biology
