Explore the complex behavior of Saccharomyces cerevisiae cells in media containing repressing and inducing carbon sources in this 92-minute conference talk from the International Centre for Theoretical Sciences. Delve into topics such as diauxie in bacteria and yeast, galactose catabolism, and the evolution of the GAL genetic switch. Examine paradoxes in long-term adaptation and unusual epistatic interactions, and investigate the discrepancies between biochemical and genetic data. Learn about polymorphisms in yeast galactose sensors, growth patterns on various sugar combinations, and gene duplication in the adaptive evolution of genetic switches. Gain insights into the constraints on galactose network induction and the complexities of nutrient decision-making in yeast.
How Do Cells of Saccharomyces Cerevisiae Behave in a Medium Containing Repressing and Including Carbon Sources
International Centre for Theoretical Sciences via YouTube
Overview
Syllabus
How do cells of Saccharomyces cerevisiae behave in a medium containing repressing and including carbon sources
Erwin Schrodinger 1944
How do cells of budding yeast behave in a medium containing repressing and inducing carbon sources?
Diauxie in Bacteria
Revisiting Diauxie in yeast
Galactose Catabolism
Evolution of GAL Genetic Switch in Yeast
S.cerevisiae GAL switch
Paradox 1: Long term adaptation
Paradox 2: Unusual Epistatic interactions in the GAL switch
"How wounderful that we have met with a paradox, Now we have some hope of making progress"
Biochemistry or Biochemist? is at odds with Genetics!
Biochemical data is clearly incompatible with the genetic data
Gal3p physically interacts with Gal80s-1p
GAL genetic switch of k.lactis Vs S.cerevisiae
Would GAL80s-1 GAL4c strain show long term adaptation?
Ultra-sensitivity of the GAL switch
Glucose repression is reduced in the mutant strain
Polygenic evolution of a sugar specialization trade-off in yeast
Polymorhisms in the yeast galactose sensor underlie a natural contium of nutrient-decision phenotypes
Diauxie variation is due to polymorphism at GAL3 locus
Growth on Glucose 0.25% + Galactose 0.25%
Growth on Melibiose-A disaccharide of Glu and Gal
Competition between the mutant and wild type
Gene duplication and the adaptive evolution of a classic genetic switch
A living vector field reveals constraints on galactose network induction in yeast
Taught by
International Centre for Theoretical Sciences
