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YouTube

High-Throughput qPCR Microfluidics Technology with Singleplex Accuracy for Plant and Animal Genomics

Labroots via YouTube

Overview

Explore high-throughput qPCR microfluidics technology with singleplex accuracy for plant and animal genomics in this 45-minute webinar presented by Dr. Naveen Ramalingam, Senior Director of Assay Development at Standard BioToolsâ„¢. Learn how microfluidics qPCR technology enables massively parallel reactions using a single color, offering sensitivity comparable to standard thermal cycler workflows while saving costs and simplifying high-throughput processes. Discover the advantages of this technology in overcoming limitations of traditional multiplex qPCR, including the ability to screen more than 10 pathogens or SNPs in a sample. Gain insights into the system's performance, its applications in plant breeding, animal health, and pathogen detection, and how it improves productivity and sustainability in various genomic studies. Understand the workflow improvements, from sample processing to result generation, and how this technology can generate 9,216 qPCR results in just 4 hours.

Syllabus

Intro
STANDARD BIOTOOLS Delivers leading life science tools
WHAT IS MICROFLUIDICS? Manipulation of liquids in small volumes (nanoliters)
Commercial fishing. Bristol Bay Alaska
IMPROVING Productivity and sustainability
LIMITATIONS OF TRADITIONAL qPCR METHODS
SAMPLE PROCESSING Traditional qPCR vs. microfluidics-based PCR
SAMPLE SCALABILITY
IMPROVEMENTS TO LAB EFFICIENCY AND CAPACITY
MULTIPLEX THROUGHPUT WITH SINGLEPLEX ACCURACY
DESIGN YOUR PANEL WITH ANY PATHOGEN
HOW ARE SAMPLES LOADED?
MICROFLUIDICS Increases throughput, lowers reagent use
X9 SYSTEM PERFORMANCE DATA
X9 REAL-TIME PCR SYSTEM Broad spectra of consumable configuration to support different experimental needs
Molecular tools for plant breeding and quality control
Effective solutions for the whole agrigenomics continuum
An integrated and cost-effective NGS workflow
DETECT MULTIPLE PATHOGENS Up to 192 targets in one test
AN INTEGRATED AND COST-EFFECTIVE PCR WORKFLOW FOR ANIMAL HEALTH
IMPROVING ANIMAL BREEDING Assessing meat quality via SNP analysis
DETECTION OF BOVINE RESPIRATORY AND ENTERIC PATHOGENS
DETECTION OF ENZOOTIC PATHOGENS IN PIGS
Conclusion

Taught by

Labroots

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