Investigating the Conditionally Essential Role of Genes Using CRISPR-Based Screens

Explore a 36-minute webinar featuring Guy Kunzmann, BS, as he delves into the conditionally essential role of genes using CRISPR-based forward genetic screens. Learn how these screens identify essential genes in proliferating human cell lines and discover the limitations of conventional media in reflecting nutrient conditions within the human body. Gain insights from Kunzmann's background in Molecular and Cellular Biology and his experience in cell culture, particularly in defining mechanisms of cell stress responses in the murine intestinal epithelium. Understand the importance of environmental factors in gene essentiality and how they influence CRISPR-based screens performed on cultured cells.