Dive into the world of cryo-electron microscopy (cryo-EM) with this comprehensive course led by Professor Grant Jensen from the California Institute of Technology. Explore the fundamental principles, techniques, and applications of cryo-EM through a series of lectures covering topics such as electron microscope anatomy, Fourier transforms, image formation, sample preparation, 3D reconstruction, tomography, single particle analysis, and 2D crystallography. Gain hands-on knowledge of the challenges and limitations in biological transmission electron microscopy, and learn about advanced techniques like energy filtering and detector technology. Master the intricacies of data collection, reconstruction workflows, and result interpretation in various cryo-EM applications. Develop a solid foundation in this cutting-edge structural biology technique over 15 hours of in-depth instruction.
Overview
Syllabus
Getting Started in Cryo-EM with Professor Grant Jensen.
Welcome to Cryo-EM - G. Jensen.
Cryo-EM Review Slides URL.
Introduction: Why Electrons? - G. Jensen.
Part 1: Basic Anatomy of the EM - G. Jensen.
Part 1: Electron Guns - G. Jensen.
Part 1: EM Lenses - G. Jensen.
Part 1: The Column - G. Jensen.
Part 1: Sample Chamber - G. Jensen.
Part 1: Energy Filters - G. Jensen.
Part 1: Detectors - G. Jensen.
Part 1: Vacuum Systems - G. Jensen.
Part 1: Summary and Safety - G. Jensen.
Part 2: Fourier Transforms for Beginners - G. Jensen.
Part 2: 1-D Sine Waves and Their Sums - G. Jensen.
Part 2: 1-D Reciprocal Space - G. Jensen.
Part 2: 2-D Waves and Images - G. Jensen.
Part 2: 2-D Transforms and Filters - G. Jensen.
Part 2: 3-D Waves and Transforms - G. Jensen.
Part 2: Convolution and Cross-Correlation - G. Jensen.
Part 3: Image Formation - G. Jensen.
Part 3: Amplitude and Phase Contrast 2016 - G. Jensen.
Part 3: Wave Propagation and Phase Shifts - G. Jensen.
Part 3: The Contrast Transfer Function - G. Jensen.
Part 3: Defocus and Its Effects - G. Jensen.
Part 3: Envelopes - G. Jensen.
Part 3: CTF Correction - G. Jensen.
Part 4: Fundamental Challenges in Biological TEM - G. Jensen.
Part 4: Sample Prep: Room Temperature Methods - G. Jensen.
Part 4: Sample Prep: Methods Involving Freezing - G. Jensen.
Part 4: Sample Prep: Grids - G. Jensen.
Part 4: 3-D Reconstruction - G. Jensen.
Part 4: Dose Limitations - G. Jensen.
Part 5: Tomography - G. Jensen.
Part 5: Tomography Data Collection and Reconstruction - G. Jensen.
Part 5: Tomography Identfying Objects of Interest - G. Jensen.
Part 5: Tomography Limitations - G. Jensen.
Part 5: Tomography Parameters and Handedness - G. Jensen.
Part 6: Single Particle Analysis - G. Jensen.
Part 6: SPA Sample Prep - G. Jensen.
Part 6: SPA Data Collection - G. Jensen.
Part 6: SPA Reconstruction Basic Workflow - G. Jensen.
Part 6: SPA Reconstruction Additional Topics - G. Jensen.
Part 6: SPA Interpretations and Limitations - G. Jensen.
Part 7: 2D Crystallography Intro and Sample Prep - G. Jensen.
Part 7: FT of a 2D Crystal - G. Jensen.
Part 7: 2D Crystallography Data Collection and Reconstruction - G. Jensen.
Part 7: Helical Tubes - G. Jensen.
Part 7: Farewell - G. Jensen.
Taught by
caltech
