Elucidating the Composition of CRISPR Cas12f1 Complexes Using Mass Photometry

Explore the composition of CRISPR-Cas12f1 complexes through mass photometry in this 36-minute webinar presented by PhD student Selgar Henkel-Heinecke. Delve into the stoichiometry of miniature effector complexes AsCas12f1 and SpCas12f1, crucial for genome engineering applications. Discover how these compact CRISPR-Cas systems interact with nucleic acids during the genome editing process, forming binary and ternary complexes. Gain insights into target recognition and DNA cleavage mechanisms, essential for improving future genome editing techniques. Learn how mass photometry analysis contributes to a deeper mechanistic understanding of these systems, paving the way for advancements in the field of genome engineering.