ELISA Test - Direct, Indirect & Sandwich

Explore the intricacies of ELISA (Enzyme-Linked Immunosorbent Assay) testing in this comprehensive lecture. Delve into the fundamental principles, procedures, and applications of Direct, Indirect, and Sandwich ELISA techniques. Learn about ELISA plates, serum coating, blocking agents, and spectrophotometry for color detection. Understand the significance of enzyme-linked antibodies and the concept of epitopes and polyclonal antibodies. Discover how Indirect ELISA improves sensitivity through color amplification with secondary antibodies. Examine the advantages of Sandwich ELISA in increasing specificity through multiple antibody detection. Compare ELISA with other immunoassays like Radio-immuno-assay (RIA) and explore its diverse applications in detecting antigens, antibodies, microbial antigens, hormones, and specific conditions like HIV exposure and pregnancy.