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DIY Scanning Laser Microscope

Breaking Taps via YouTube

Overview

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Learn how to build a 3D printed scanning laser confocal microscope for collecting 3D surface topology and profilometry data in this 22-minute video tutorial. Explore the differences between confocal and widefield microscopy, understand the OpenFlexure motion platform, and delve into the confocal optical breakdown. Discover the intricacies of the delta motion stage, photodiode amplifier, and confocal pinhole demonstration. Compare camera and photodiode usage, consider data processing challenges, and examine the resulting images. Gain insights into potential optimizations for improving microscope performance and image quality.

Syllabus

Intro
Confocal vs Widefield Microscopy
OpenFlexure Motion Platform
Confocal optical breakdown
Delta motion stage
Photodiode amplifier
Confocal Pinhole demonstration
Camera vs Photodiode
Data processing considerations
Images and results!
Optimizations
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Taught by

Breaking Taps

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