The course will be a short primer to understand how ‘animal cell culture technologies’ have strengthened the bio-medical research from basic research to the modern drug discovery. Animal cell culture was first performed in the very first decade of the 19th century. Since then, tremendous development has taken place in this field. The lectures will help the researcher to appreciate the developments during the last hundred years and will help them to independently set up cell culture laboratories. For non-biologist, it will be an informal way to demystify the intriguing routes of biomedical research where cell culture is a very ‘potent tool’.
Overview
Syllabus
Introduction of Cell Culture Technology.
Philosophy and complexity in cell culture.
To grow the cell outside the body.
Cell cycle concept.
Dividing cells.
Biology of cell culture.
Layout(s) & design(s) of cell culture facility.
Precautions during designing the lab layout - I.
Precautions during designing the lab layout - II.
Precaution during designing the lab out - III.
State of the art facility in cell culture lab - I.
State of the art facility in cell culture lab - II.
Specialized facility in cell culture lab.
Interaction of cell and glass/polycorbonate surface - II.
Lecture 15_2017.
Poly D lysine deposition.
Surface chemical analysis.
Cell growth process.
Cell surface interface.
Cell culture substrate patterning.
Introduction of define system.
Mechanical dissociation of hippocampal tissue.
Rules for mechanical dissociation of tissue.
Drum molecule testing.
Adult hippocampal neuron dissociation.
Cell separation & In vitro myelination cell culture mode - I.
Cell separation & In vitro myelination cell culture mode - II.
Cell separation & In vitro myelination cell culture mode - III.
Cell separation & In vitro myelination cell culture mode - IV.
Cell separation & in vitro myelination cell culture mode - V.
Fluorescent assisted cell sorting.
Condition for regenerated cells.
Introduction of skeletal muscle cell culture.
Skeletal muscle cell culture.
Cardiac muscle cell culture.
Advance cell culture modules - I.
Advance cell culture modules - II.
Advance cell culture modules - III.
Advance cell culture modules - IV.
Advance cell culture modules - V.
Taught by
Cell Culture Technologies
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Reviews
5.0 rating, based on 1 Class Central review
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Martha JAIt was good. I am grateful for attended this course, from basic thing included the laboratory to complex culture cells.
It will be very useful for me.
I will share this information with my partners.
