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Explore the groundbreaking Biosphere 2 facility in this comprehensive talk by John Allen, William Dempster, and Mark Nelson. Delve into the challenges and achievements of the world's largest biospherics laboratory during its closed ecological system experiments from 1991 to 1994. Learn about the conceptual design, including innovative bioregenerative techniques for air and water purification, nutrient recycling, and sustainable agriculture. Discover the relevance of Biosphere 2 research for improving global ecosystem management and ecological restoration. Examine striking atmospheric and system dynamics, gaining insights into whole system metabolism and complex non-linear systems modeling. Gain a unique perspective on the "biospherian" experience of living and working within a closed biospheric system, and understand the implications for future closed system designs and our understanding of Earth's biosphere.