Overview
Syllabus
Intro
Bacteriological Diagnosis of Tuberculosis: Smear and Culture
Diagnosis of TB
Good quality sputum / gastric aspirate
Triple Packaging of Infectious Material
Conventional Bacteriological diagnostic methods for TB diagnosis
Appearance of TB bacilli in smears
Limitations of Smear Microscopy
Light Emitting Diode (iLED) microscope
Solid Culture - Egg based Lowenstein Jensen & Agar Media
Limitations of solid culture for TB diagnosis
Features of MGIT960
Components and Principle MGIT960
Identification and confirmation of M.tb growth in liquid culture
BACTEC MGIT960 - Result output
Disadvantages of MGIT 960
Species Identification of NTM Biochemical Tests
Molecular testing
Whole genome Sequencing: NTM
Key messages
Taught by
NPTEL-NOC IITM