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Explore the fundamentals and clinical applications of flow cytometry in acute leukaemias through this comprehensive lecture. Begin with an in-depth examination of basic principles and plot interpretation techniques, laying a solid foundation for understanding this crucial diagnostic tool. Progress to an extensive discussion on the practical use of flow cytometry in clinical settings, with a particular focus on its relevance to acute leukaemias. Gain insights into how flow cytometry results can inform and complement morphological reviews, enhancing diagnostic accuracy and patient care. This 1 hour and 35 minute presentation, delivered by Dr Timothy Farren for The Royal College of Pathologists, offers valuable knowledge for pathologists, hematologists, and medical professionals involved in the diagnosis and management of acute leukaemias.