细胞生物学是生命科学重要的基础学科之一，是分子生物学与个体生物学之间承上启下的学科。本课程主要是让学生了解细胞的基本结构及其功能，熟悉细胞生命活动的基本特点及其分子机制，并了解当前细胞生物学的发展动态和国际前沿知识。本课程为中英文双语教学，即授课用中文，而PPT，教材及考试都为英文。适合对生物感兴趣，或者立志将来从事生命科学研究的学生进行学习。
Overview
Syllabus
- 1.Introduction of Molecular cell biology
- 1.1The Objectives and development of cell biology
- 1.2 Microscope and cell theory
- 1.3 Major evens in cell biology
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- 2.Culturing and visualizing cells
- 2.1 Cell culture
- 2.2 Monoclonal antibody
- 2.3 Fluorescence Microscopy
- 2.4 Flow cytometry
- 2.5 pinning Disk Confocal Microscopy
- 2.6 FRAP and FRET
- 2.7 RNA interference
- 2.8The structure of CRISPR cas9
- 2.9 CRISPR mechanism
- 3.Biomembrane Structure
- 3.1. The structrures of the plasmic membrane
- 3.2. Composition and structural organization
- 3.3. Forms of lipid molecules exist in water
- 3.4. Lipids movement in biomembrane
- 3.5. Structure of Membrane Proteins
- 3.6 Synthesis of Phospholipids and Intracellular Movement
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- 4.Transmembrane transport of Ions and small molecules
- 4.1 Overview of transmembrane transport
- 4.2 Facilitated Transport of Glucose and Water
- 4.3 ATP-powered pumps
- 4.4 Nak pump
- 4.5 Ca2+ pump
- 4.6 K+ channel and the Resting membrane potencial
- 4.7 Cotransport by Symporters and Antiporters
- 4.8 Transcellular transport
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- 5.Cellular Energetics
- 5.1 Overview of aerobic oxidation
- 5.2 structure of Mitochondria
- 5.3 Mitochondrial Oxidation of Fatty Acids Generates ATP
- 5.4 The Electron Carriers and Electron Transport Chain
- 5.5 The Electron Transport and Generation of the Proton-Motive Force
- 5.6 Chemiosmotic theory and Endosymbiont hypothesis
- 5.7 Harnessing the Proton-Motive Force to synthesize ATP
- 5.8 Electron Transport and Reactive Oxygen Species (ROS)
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- 6.Moving Proteins into Membranes and Organelles
- 6.1 Targeting Proteins to and Across the ER Membrane
- 6.2 Insertion of single-pass proteins into the ER
- 6.3 Insertion of Multipass Proteins into the ER
- 6.4 Protein Glycosylation and Folding in the ER
- 6.5 Formation of disulfide bonds in the ER
- 6.6 Protein Folding Quality Control in the ER
- 6.7 Targeting of proteins to Mitochondria
- 6.8 Targeting of Peroxisomal Proteins
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- 7.Vesicular traffic,secretion and endocytosis
- 7.1 Molecular Mechanisms of Vesicle Budding and Fusion
- 7.2 Early Stages of the Secretory Pathway
- 7.3 Later Stages of the Secretory Pathway
- 7.4 Receptor-Mediated Endocytosis
- 7.5 Directing Membrane Proteins and Cytosolic Materials to the Lysosome
- 7.6 The structure and functions of Lysosomes
- 7.7 Exosome
- 7.8 Cell Penetrating Peptides
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- 8.Signal transduction and G protein-coupled receptors
- 8.1 Overview of Cell Communication
- 8.2 Signaling Molecules and Receptors~1
- 8.3 G Proteins and G Protein-coupled Receptors (GPCR)
- 8.4 GPCR Mediated Signaling Pathways
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- 9.Signaling pathways that control gene activity
- 9.1 Overview of signaling pathways that control gene activity
- 9.2 The RTK-Ras-MAPK and JAK-STAT Signaling Pathway
- 9.3 Phosphoinositide Signaling Pathways
- 9.4 TGF-b Signaling Pathways
- 9.5 Signaling Pathways controlled by Ubiquitination
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- 10.Cell organization and movement I Microfilaments
- 10.1 Introduction of cytoskeleton
- 10.2 Dynamic Assembly of Actin Filaments
- 10.3 Actin-based Cellular Structures
- 10.4 Myosins and Myosin-Powered Movements
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- 11. Cell organization and movement II Microtubules and intermediate filament
- 11.1Microtubule Structure and Organization
- 11.2 Microtubule Dynamics
- 11.3 Regulation of Microtubule Structure and Dynamics
- 11.4 Kinesins & Dyneins Microtubule-based Motor Proteins
- 11.5 Cilia & Flagella Microtubule-based Surface Structures
- 11.6 Mitosis Mitotic Spindle
- 11.7 Intermediate Filaments
- 11.8, Microtubule Structure and Organization
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- 12.Nucleus, chromatin and chromosomes
- 12.1 Nuclear Envelope (NE), Nuclear Pore Complex (NPC) and Nuclear Transport
- 12.2 Structural Organization of Eukaryotic Chromosomes
- 12.3 Morphology & Functional Elements of Eukaryotic Chromosomes
- 12.4 Nucleolus
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- 13.The Eukaryotic Cell Cycle
- 13.1 Overview of the Cell Cycle and its Control
- 13.2 Model Organisms and Methods to Study the Cell Cycle
- 13.3 CDK and Its Discovery
- 13.4 Regulation of CDK Activity
- 13.5 Commitment to the Cell Cycle and DNA Replication
- 13.6 Entry into Mitosis and Completion of Mitosis
- 13.7 Surveillance Mechanisms in Cell Cycle Regulation
- 13.8 Meiosis A Special Type of Cell Division
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- 14.Integrating cells into tissues
- 14.1 An Overview on Cell-Cell and Cell-Matrix Adhesion
- 14.2.1 Type of Cell Junctions and Anchoring Junctions
- 14.2.2 Tight junctions and Gap junctions
- 14.3 ECMs of The Basal Lamina
- 14.4 ECMs of Connective tissue
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- 15.Stem cells, cell asymmetry
- 15.1 Early metazoan developmnt and embryonic stem cells
- 15.2 Stem Cells and Niches in Multicellular Organisms
- 15.3 Mechanisms of cell polarity and asymmetric cell division
- 15.4 Cell death and its regulation
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- 期末考试
Taught by
Ye Jun, Han Jiahuai, Yu Xianwen, Jin Quanwen, and Mo Pingli
