Metabolic Engineering

Overview

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Metabolic engineering is an emerging field of biotechnology/bioprocess engineering which aims towards purposeful modification of cellular (metabolic, gene regulatory, and signalling) processes/networks to achieve desirable goals such as enhanced production of metabolites including pharmaceuticals, biofuels and biochemicals and other biotechnology products. This course aims to provide fundamental and advanced knowledge in the development of microbial strain for bio production through metabolic engineering.as INTENDED AUDIENCE :B Tech (Biotechnology/Bioengineering); B Sc. (Microbiology, Biotechnology, Botany); M Sc. (Biotechnology / Life Sciences)PREREQUISITES : Basics of Microbiology, Biochemistry, GeneticsINDUSTRIES SUPPORT :None

Syllabus

Week 1:Introduction to Metabolic Engineering, Basic concepts; Scopes and Applications; Metabolism overview _1 (Cellular Transport processes, Fueling Reactions)Week 2:Cellular Metabolism Overview_2 (Biosynthetic reactions, Polymerization, Growth Energetics); Regulation of Metabolic PathwaysWeek 3: Reconstruction of Genome-scale metabolic network Week 4:Examples of pathway manipulations by metabolic engineering : Ethanol, Aminoacids, antibiotics, vitamines, biopolymers, etc.
Week 5:Examples of pathway manipulations by metabolic engineering : Improvements of cellular properties, Biodegradation, Week 6:Metabolic Flux Analysis: Flux Balance Analysis (FBA), Flux Variability Analysis, Flux MapWeek 7:Experimental determination of Metabolic Fluxes: Isotope labeled substrate, Isotope mapping Mapping Matrix, Isotope Distribution VectorWeek 8:Application of metabolic Flux Analysis