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NPTEL

Experimental Nanobiotechnology

NPTEL via Swayam

Overview

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ABOUT THE COURSE:Nanobiotechniques include a versatile combination of practical sessions from different spectra of nanotechnology. The session consists of the emerging applications of nanoparticles including biosensors, nanofiber scaffolds, 3D bioprinting, nanotoxicology and various characterization techniques. The increased use of nanotechnology in various sectors especially for biomedical applications makes it inevitable to be included in academic sessions. The basic knowledge of nanobiotechnology including nanoparticle synthesis and its characterization paved the way for the development of better platforms in the biomedical field. The current course opens a new arena for the students to get practical knowledge in the field of nanobiotechnology.INTENDED AUDIENCE:UG/PG students of Nanotechnology / Biotechnology and relevant disciplinesPREREQUISITES: Basic knowledge in biology / nanotechnology Or Desirable: NPTEL course on Biomedical nanotechnologyINDUSTRY SUPPORT: Health care and Pharma companies

Syllabus

Week 1:Physical method of nanoparticle synthesis: Ball milling, Chemical synthesis of nanoparticles: Gold Nanoparticles, Hydrothermal synthesis of Carbon Dots, Polymeric nanoparticle synthesis (Albumin & PLGA Nanoparticles), Hydrogel fabricationWeek 2:Nanoparticle characterization techniques: UV-Vis Spectroscopy, DLS- Hydrodynamic Size & Zeta potential, Fourier transform infrared spectroscopy, Fluorescence Spectroscopy, Electron microscopic analysisWeek 3:Biomedical applications: Nanobiosensor, Fabrication of nanofibers using electrospinning, Hemocompatibility test, 3D Bioprinting, In vitro 3D cell culture. Week 4:Nanotoxicology: Antibacterial properties of nanomaterials, In vitro cytotoxicity analysis, Apoptotic studies, In vivo toxicity studies using zebrafish embryo and hydra.

Taught by

Prof. P. Gopinath

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