This course is an advanced treatment of biochemical mechanisms that underlie biological processes. Topics include macromolecular machines such as the ribosome, the proteasome, fatty acid synthases as a paradigm for polyketide synthases and non-ribosomal polypeptide synthases, and polymerases. Emphasis will be given to the experimental methods used to unravel how these processes fit into the cellular context as well as the coordinated regulation of these processes.
Overview
Syllabus
1. Introduction to Biological Chemistry II.
2. Protein Synthesis 1.
R1. Determining, Analyzing, and Understanding Protein Structures.
3. Protein Synthesis 2.
4. Protein Synthesis 3.
5. Protein Synthesis 4.
R2. Pre-Steady State and Steady-State Kinetic Methods Applied to Translation.
6. Protein Synthesis 5.
7. Protein Synthesis 6.
8. Protein Folding 1.
R3. Pre-Steady State and Steady-State Kinetic Methods Applied to Translation.
9. Protein Folding 2.
10. Protein Folding 3.
11. Protein Folding 4.
R4. Purification of Native and Mutant Ribosomes, Protein Purification.
12. Protein Degradation 1.
13. Protein Degradation 2.
14. Protein Degradation 3.
R5. Overview of Cross-Linking, Including Photo-Reactive Cross-Linking Methods.
15. PK and NRP Synthases 1.
16. PK and NRP Synthases 2.
R6. Macromolecular Electron Microscopy Applied to Fatty Acid Synthase.
17. PK and NRP Synthases 3.
18. PK and NRP Synthases 4.
R7. Application of Single Molecule Methods.
19. Cholesterol Biosynthesis 1.
20. Cholesterol Biosynthesis 2.
21. Cholesterol Biosynthesis 3 & Cholesterol Homeostasis 1.
R8. Application of CRISPR to Study Cholesterol Regulation.
22. Cholesterol Homeostasis 2.
23. Cholesterol Homeostasis 3.
24. Cholesterol Homeostasis 4.
R9. Cholesterol Homeostasis and Sensing.
25. Cholesterol Homeostasis 5 & Metal Ion Homeostasis 1.
26. Metal Ion Homeostasis 2.
27. Metal Ion Homeostasis 3.
R10. Metal-Binding Studies and Dissociation Constant Determination.
28. Metal Ion Homeostasis 4.
29. Metal Ion Homeostasis 5.
R11. Mass Spectrometry.
30. Metal Ion Homeostasis 6.
31. Metal Ion Homeostasis 7 & Reactive Oxygen Species 1.
R12. Mass Spectrometry of the Cysteine Proteome.
32. Reactive Oxygen Species 2.
33. Reactive Oxygen Species 3.
34. Reactive Oxygen Species 4 & Nucleotide Metabolism 1.
R13. Fluorescence Methods.
35. Nucleotide Metabolism 2.
36. Nucleotide Metabolism 3.
Taught by
Prof. JoAnne Stubbe and Prof. Elizabeth Nolan
