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Early zebrafish embryos as an experimental paradigm to understand cell size scaling during embryonic development
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The Logistics of Shaping a Vertebrate Embryo in Developmental Space and Time by Sreelaja Nair
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- 1 The logistics of shaping a vertebrate embryo in developmental space and time
- 2 Everything complex is simple to begin with..
- 3 The "teenage angst" of embryos
- 4 Early zebrafish embryos as an experimental paradigm to understand cell size scaling during embryonic development
- 5 Cells decrease in size during early zebrafish development
- 6 Cell size decrease during early zebrafish development
- 7 Dynamically evolving cell sizes are distributed in conserved proportions during early development
- 8 Why study cell size during early embryonic development?
- 9 How does one change cell size when one does not know what regulates cell size?
- 10 Generation of zebrafish haploids and tetraploids
- 11 Cell sizes in haploid and tetraploid zebrafish embryos are altered in comparison to diploid embryos
- 12 Haploid and tetraploid embryos generate cell collectives of altered sizes
- 13 Movement is an inherently complex phenomenon
- 14 Gastrulation is the phenomenon of en mass cell migration in animal embryos,
- 15 Model organisms allow us to study evolutionary commonalities between species
- 16 The conundrum
- 17 Early cell divisions in embryos result in constantly shrinking cell sizes to generate a cell mass
- 18 Generated zebrafish haploids and tetraploids to create embryos made of altered cell sizes
- 19 Haploid and tetraploid embryos are delayed in epiboly initiation, progression and gastrulate abnormally
- 20 Haploids and tetraploids embryos have defects in anterior migration,
- 21 Haploid and tetraploid embryos generate cell collectives of altered sizes
- 22 Dynamically evolving cell sizes are distributed in conserved proportions during early development
- 23 Movement in an embryological context always gives rise to form
- 24 Haploids and tetraploids embryos have defects in anterior migration,
- 25 If cells in diploid embryos are made to be of suboptimal sizes,
- 26 If smaller cells are induced to achieve normal cell size optima,
- 27 Do sub-optimally sized cells migrate aberrantly during gastrulation?
- 28 Do sub-optimally sized cells migrate aberrantly during gastrulation? - Yes
- 29 The migratory behaviour of cells in a suboptimal sized cell collective is altered
- 30 Do sub-optimally sized cells migrate aberrantly during gastrulation? Analysing cells in an embryo by generating mosaic embryos
- 31 Smaller or larger cells continue to migrate aberrantly during gastrulation,
- 32 Migration is influenced by environment of the cell collective
- 33 Why does a cell of suboptimal size migrate poorly? Assaying membrane action dynamics during gastrulation in live embryos
- 34 Membrane protrusion dynamics change when cell size changes
- 35 Membrane protrusion dynamics of action containing protrusions change when cell size changes
- 36 Quick recap...
- 37 Why is this interesting, besides it just being interesting?
- 38 Endodermal organogenesis and function is also compromised
- 39 The big question: How do embryos set up cell size optima?
- 40 The logistics of shaping a vertebrate embryo in developmental space and time
- 41 Vertebrate Embryogenesis Group
