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Testing the Enzymes
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Classroom Contents
Synthetic Biology and Materials Science - Nature-Made Plastics
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- 1 Intro
- 2 we can select a series of enzymes from a catalog that will produce some desired industrial compound
- 3 petroleum/crude oil
- 4 methyl methacrylate (MMA)
- 5 advantages of PMMA
- 6 production of PMMA from crude oil is not sustainable
- 7 oil in the ground will eventually run out
- 8 Project Lead: Aaron Korkegian
- 9 a-methylene-y-butyrolactone (MBL)
- 10 fermentation host (yeast, bacteria, etc.)
- 11 enzyme database + efficient algorithm
- 12 natural substrate
- 13 the methyl may clash in the active site
- 14 Proposed Pathway
- 15 Generating the Enzymes
- 16 Testing the Enzymes
- 17 Optimizing the Enzymes
- 18 Testing the Optimized Enzymes
- 19 Confirmed Pathway
- 20 we can modify the ribosomal binding site sequences for any of the enzymes
- 21 any such reaction can be run in precisely the same large vat with the same material
- 22 PROFESSOR DAVE EXPLAINS
