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Topo cloning
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Classroom Contents
Recombinant DNA Technology
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- 1 DNA cloning
- 2 Plasmid isolation protocol
- 3 DNA microarray
- 4 DNA ligase mechanism
- 5 Role of alkalyne phosphaase in molecular cloning
- 6 Role of linker and adaper DNA
- 7 PCR types
- 8 Genomic library screening
- 9 PCR (polymerase chain reaction) in detail
- 10 Southern blot protocol
- 11 Western blot
- 12 Northern blot
- 13 Southern blot
- 14 Expression vector
- 15 Cloning vector
- 16 Genomic library
- 17 cDNA library
- 18 PCR optimization | PCR technique
- 19 Yeast artificial chromosome (YACs)
- 20 Plasmid as a cloning vector
- 21 Cosmid vector
- 22 Control of gene expression in pET vector
- 23 Difference between genomic DNA library and cDNA library
- 24 Construction of genomic library using yeast artificial chromosome
- 25 Construction of genomic library using lamda phage
- 26 Construction of genomic library using cosmids
- 27 Blue white screening of DNA clones
- 28 Using oligonucleotide probe to screen clones from genomic DNA library
- 29 Replica plating
- 30 Immunological screening (genomic library screening method)
- 31 Expression screening (genomic library screening)
- 32 Colony hybridization assay
- 33 Synthesis of probes for Southern blotting
- 34 Southern Blotting overview
- 35 Real time PCR
- 36 Real time PCR overview
- 37 Real time PCR using SYBR green probe
- 38 Real time PCR using scorpion primer
- 39 Nested PCR
- 40 Inverse PCR
- 41 Hot start PCR
- 42 Colony PCR
- 43 Real time PCR using taqman dye
- 44 Real time PCR using molecular beacon
- 45 Quantitative real time PCR (qPCR)
- 46 Expression vector, pET vector
- 47 Expression vector, pBAD vector
- 48 Multiplex PCR
- 49 M13 vector, a type of viral vector for cloning
- 50 Cloning vector (lamda phage vector)
- 51 Genomic library screening: differential screening
- 52 Bacterial artifical chromosome (BACs)
- 53 Southern blotting applications
- 54 Properties of restriction enzymes
- 55 DNA methylation
- 56 Topo cloning
- 57 Restriction enzyme digestion (Isochizomer, neoschizomer and isocaudamer)
- 58 Difference between linker and adaptor DNA
- 59 Star activity
- 60 Error prone PCR
- 61 Degenerate PCR
