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One-Step qPCR Master Mix for Amplification from Crude or Purified Samples
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- 1 Intro
- 2 Using IDT primers, probes and master mix products for tracking SARS-CoV-2 via qPCR
- 3 Advantages of using the IDT PrimeTime™ 1-Step 4X Broad-Range Master Mix
- 4 PrimeTime One-Step 4X Broad-Range Master Mix Building Blocks
- 5 IDT Prime Time™ 1-Step 4X Broad-Range Master Mix has high endpoint fluorescence
- 6 IDT PrimeTime™ 1-Step 4X Broad-Range Master Mix provides excellent multiplexed limit of detection (LOD) when using purified RNA as template
- 7 Better inhibitor resistance: heparin, hematin, humic acid
- 8 Extraction-less direct amplification capability
- 9 IDT PrimeTime™ 1-Step 4X Broad-Range Master Mix results in comparable amplification of viral analytes between extracted and non-extracted human samples
- 10 IDT PrimeTime™ 1-Step 4X Broad-Range Master Mix provides lower Ct and higher endpoint fluorescence from human saliva and nasopharyngeal matrix as compared to competitors
