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Confocal Microscopy
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Confocal Microscopy and Its Applications in Biomolecular Diffusion Studies - Lecture 1
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- 1 Confocal Microscopy
- 2 The Size of Things
- 3 History of Microscopes
- 4 History of Microscopes
- 5 History of Microscopes
- 6 History of Microscopes
- 7 Modern Microscope
- 8 Modern Microscopy
- 9 Laser Scanning Confocal Microscopy
- 10 Laser Scanning Confocal Microscopy
- 11 Laser Scanning Confocal Microscopy
- 12 Laser Scanning Confocal Microscopy
- 13 Diffusion of Biomolecules in Vesicle Coated RNA-Based Coacervates
- 14 Activities in cell can be compartmentalized by phase separation
- 15 Diffusion of biomolecules indicates dynamic structure
- 16 PolyU/spermine coacervate system
- 17 Partitioning in coacervate
- 18 Concentration in the droplet can be calculated using microscopy
- 19 Partitioning in PolyU/spermine coacervate system
- 20 Partitioning in PolyU/spermine coacervate system
- 21 Partial Diffusion - What is Fluorescence Recovery After Photobleach FRAP?
- 22 Entire Droplet Diffusion- FRAP
- 23 FRAP Curve and equations
- 24 Partial droplet bleaching RNA recovery in polyU/spermine coacervate
- 25 Partial droplet bleaching RNA recovery in polyU/spermine coacervate
- 26 Entire droplet bleaching RNA recovery in polyU/spermine coacervate
- 27 Entire droplet bleaching RNA recovery in polyU/spermine coacervate
- 28 Vesicle assembly around coacervate droplets
- 29 Vesicle assembly around coacervate droplets
- 30 RNA diffusion in vesicle coated coacervate droplets
- 31 Conclusions
