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Optimizing subtomogram averaging
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High Throughput, High Performance Algorithms for High Resolution Subtomogram Averaging
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- 1 Intro
- 2 Structures of membrane proteins
- 3 Excitation-contraction coupling in skeletal muscle
- 4 Sample purification workflow
- 5 Structure of RyR1 in native membranes
- 6 Structure of RyR1 at the resolution of secondary structure
- 7 Comparison to the other reported structures of RyR1
- 8 Subtomogram averaging is successful for a class of molecules
- 9 Throughput considerations
- 10 More options for particle picking with user assistance
- 11 Benchmarking tomoBEAR
- 12 Optimizing subtomogram averaging
- 13 Substack analysis with SUSAN
- 14 CTF estimation module in SUSAN - "normalization"
- 15 CTF estimation module in SUSAN - "linearization"
- 16 3D reconstruction in SUSAN
- 17 Benchmarking SUSAN
- 18 Conclusions and outlook
