Metagenomics Taxonomic Classification Using Kraken 2 in OmicsBox

Metagenomics Taxonomic Classification Using Kraken 2 in OmicsBox

bioinformagician via YouTube Direct link

Trimming reads using Trimmomatic

13 of 21

13 of 21

Trimming reads using Trimmomatic

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Metagenomics Taxonomic Classification Using Kraken 2 in OmicsBox

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  1. 1 Intro
  2. 2 Metagenomics approaches
  3. 3 Overview of metagenomics workflow steps
  4. 4 Questions metagenomics analysis help to answer?
  5. 5 Case study and study design
  6. 6 About BioBam
  7. 7 Exploring OmicsBox
  8. 8 Downloading metagenomics data
  9. 9 Performing QC on raw reads using FastQC
  10. 10 Assessing FastQC report in OmicsBox
  11. 11 QC Fail: Per Sequence GC Content
  12. 12 QC Fail: Per Base Sequence Content
  13. 13 Trimming reads using Trimmomatic
  14. 14 Assessing Trimmomatic report in OmicsBox
  15. 15 Post-trimming QC
  16. 16 Performing Taxonomic classification using Kraken 2
  17. 17 Exploring Kraken 2 report
  18. 18 Krona Charts to visualize taxonomic classification
  19. 19 Principle Coordinate Analysis
  20. 20 Stacked bar charts to visualize relative abundances of taxa
  21. 21 Differential Abundance Analysis of taxa

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