Inference Methods for High-Throughput CRISPR Screens - CGSI 2022

Inference Methods for High-Throughput CRISPR Screens - CGSI 2022

Computational Genomics Summer Institute CGSI via YouTube Direct link

Intro

1 of 13

1 of 13

Intro

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Inference Methods for High-Throughput CRISPR Screens - CGSI 2022

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  1. 1 Intro
  2. 2 How can so many genes contribute to complex traits?
  3. 3 Upstream regulators can be inferred by perturbations
  4. 4 Fluorescence activated cell sorting (FACS) + CRISPR enable high-throughput gene expression screening
  5. 5 Review of setup from the computational side
  6. 6 Start with the question
  7. 7 We have unique data - let's think carefully
  8. 8 Multiple guides target the same gene and thus should be correlated
  9. 9 What is my sampling distribution?
  10. 10 The model is a form of density estimation with overdispersion
  11. 11 Our updated model links the unobserved reported to the sampling distribution
  12. 12 Our new model incorporates sparsity at the gene- level
  13. 13 Hierarchical model enables accurate inference with few samples

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