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Find Markers and Cluster Identification in Single-Cell RNA-Seq Using Seurat - Workflow Tutorial
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- 1 Intro
- 2 findMarkers, findAllMarkers, findConservedMarkers
- 3 Study design
- 4 Load data
- 5 Visualize by clusters and condition
- 6 findAllMarkers
- 7 DefaultAssay 'RNA'
- 8 findConservedMarkers for cluster 3
- 9 Visualize canonical markers in a FeaturePlot
- 10 RenameIdents
- 11 Annotating clusters and marker databases
- 12 Annotating rest of the clusters
- 13 Perform differential expression in CD16 Monocytes between conditions findMarkers
- 14 Visualize markers identified by findConservedMarkers vs findMarkers