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Classroom Contents
DESeq2 Basics Explained - Differential Gene Expression Analysis - Bioinformatics 101
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- 1 Intro
- 2 A typical study design
- 3 Features of RNA-Seq counts data
- 4 Poisson distribution for counts data
- 5 Why is Poisson not the best model?
- 6 Negative Binomial is the way to go!
- 7 DESeq2 steps
- 8 Biases in counts data
- 9 Estimate Size Factor median of ratios method
- 10 Estimate Dispersions
- 11 Generalized Linear Models
- 12 Hypothesis testing
