Comprehensive Guide to Downstream Analysis for Single-Cell ATAC-Seq in R - scATAC-Seq Workflow

Comprehensive Guide to Downstream Analysis for Single-Cell ATAC-Seq in R - scATAC-Seq Workflow

bioinformagician via YouTube Direct link

Strategies to annotate scATAC-Seq cells

3 of 16

3 of 16

Strategies to annotate scATAC-Seq cells

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Comprehensive Guide to Downstream Analysis for Single-Cell ATAC-Seq in R - scATAC-Seq Workflow

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  1. 1 Intro
  2. 2 scATAC-Seq Analysis Workflow
  3. 3 Strategies to annotate scATAC-Seq cells
  4. 4 Dataset and requirements for demonstration
  5. 5 Starting with pre-processed scATAC-Seq
  6. 6 What is a gene activity matrix?
  7. 7 Creating a gene activity matrix
  8. 8 Visualizing gene activity of canonical markers
  9. 9 Visualizing cell annotations in scRNA-Seq
  10. 10 Integrating scATAC-Seq with scRNA-Seq
  11. 11 Transfer labels from scRNA-Seq to scATAC-Seq
  12. 12 Visualizing scATAC-Seq after integration
  13. 13 Performing differential accessibility analysis
  14. 14 Extracting fold changes for differentially accessible regions
  15. 15 Visualizing genomic regions of interest
  16. 16 Create interactive shiny genomic browser to visualize regions of interest

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