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Characterization of Anti-HER2 Antibodies Using Advanced Flow Cytometry and BLI Label-Free Detection
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- 1 Intro
- 2 iQue® 3: Advanced Flow Cytometry Platform
- 3 Octet BLI Label-Free Detection: Dip and Read Biosensors
- 4 Types of Information Generated Using the Octet Platform
- 5 Biosensor Chemistries
- 6 Project Workflow
- 7 Antibody Binding Kinetics
- 8 Live-cell Competition Binding
- 9 Competition Between Anti-HER2 Antibodies
- 10 Relative Glycosylation of Anti-HER2 Antibodies
- 11 iQue® Antibody Internalization Kit
- 12 Measuring Cytotoxic ADC Payload Induced Killing
- 13 iQue® Human Antibody-Dependent Cellular Phagocytosis (ADCP) Kit
- 14 ADCP Kit Gating Strategy
- 15 Comparing NK ADCC Activity Between Anti-HER2 Antibodies
- 16 Wrap Up and Perspective
