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Role of 14-3-38
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Cargo Transport in Vivo - How Is Transport Regulated? by Steven P. Gross
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- 1 Kinesin & Dynein essential for neuronal function
- 2 And for neurotropic viruses such as herpes
- 3 In this talk, I'll start with a brief
- 4 The basics of the in vitro assay: Optical Trap -Measure Motor Motility Velocity, Travel
- 5 Can also measure forces with the Optical Trap
- 6 Force production
- 7 Now, switch to in vivo -how many motors work together? use the optical trap
- 8 The optical trap in action
- 9 There are multiple types of regulation of transport:
- 10 First example-Global regulation of kinesin-family motors:
- 11 CK2/Kinesin Interaction Involved in in vivo Lipid Droplet Force Production
- 12 Second example-Semi-Global regulation of kinesin-family motors:
- 13 The increased LD-mito contacts are to allow lipid transfer to Mitos for energy production.
- 14 Might cargoes increase their ability to escape from the optical trap
- 15 Properties of adaptation....
- 16 Hypothesis: adaptation directly targets dynein via regulation- change single-molecule properties
- 17 When nuclear migration fails, it can be catastrophic
- 18 Back to cells: Test importance of NudE and Lis1
- 19 Conclusion: 1. Cargos are "smart"-they have local adaptation of force production,
- 20 Role of 14-3-38
- 21 Summary
- 22 Conclusion: Mitochondria
- 23 Lipid droplets move to contact bacteria
- 24 Lipid Droplet transport: open questions
- 25 Who did the work?
