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3D Cell Migration Using a Microfluidic Endothelium-on-a-Chip Model
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- 1 Intro
- 2 Human Tissue Models for better therapies
- 3 Meet the OrganoPlate
- 4 OrganoPlate® family
- 5 Immune cell migration
- 6 Need of novel in vitro immune migration models
- 7 Based on publication
- 8 Formation of HMEC-1 vessels in the OrganoPlate 3-lane
- 9 HMEC-1 vessels can be maintained in immune cell medium
- 10 Visualization and quantification of T cells in microfluidic chip
- 11 Perfusion of HMEC-1 vessels with fluorescently labelled T cells
- 12 ET cells and vasculature interplay
- 13 Generation of chemokine gradients in the OrganoPlate 3-lane
- 14 T cell extravasation in the presence of chemokine gradients
- 15 Donor-to-donor differences are preserved
- 16 Induction of vascular inflammation Upregulation of adhesion molecules
- 17 Blocking extravasation of T cells
- 18 Immune infiltration in the tumor compartment
- 19 Immune migration assays are also compatible with HUVECS
- 20 The OrganoPlate as a tool to study immune cell extravasation and infiltration
- 21 Cell migration in the OrganoPlate
