Find Markers and Cluster Identification in Single-Cell RNA-Seq Using Seurat - Workflow Tutorial

Find Markers and Cluster Identification in Single-Cell RNA-Seq Using Seurat - Workflow Tutorial

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Intro

1 of 14

1 of 14

Intro

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Find Markers and Cluster Identification in Single-Cell RNA-Seq Using Seurat - Workflow Tutorial

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  1. 1 Intro
  2. 2 findMarkers, findAllMarkers, findConservedMarkers
  3. 3 Study design
  4. 4 Load data
  5. 5 Visualize by clusters and condition
  6. 6 findAllMarkers
  7. 7 DefaultAssay 'RNA'
  8. 8 findConservedMarkers for cluster 3
  9. 9 Visualize canonical markers in a FeaturePlot
  10. 10 RenameIdents
  11. 11 Annotating clusters and marker databases
  12. 12 Annotating rest of the clusters
  13. 13 Perform differential expression in CD16 Monocytes between conditions findMarkers
  14. 14 Visualize markers identified by findConservedMarkers vs findMarkers

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